Colorimetric Method for Determination of Sugars and Related Substances
نویسنده
چکیده
Simple sugars, oligosaccharides, polysaccharides, and their derivatives, including the methyl ethers with free or potentially free reducing groups, give an orangeyellow color w-hen treated with phenol and concentrated sulfuric acid. The reaction is sensitive and the color is stable. By use of this phenol-sulfuric acid reaction, a method has been developed to determine submicro amounts of sugars and related substances. In conjunction with paper partition chromatography the method is useful for the determination of the composition of polysaccharides and their methyl derivatives.
منابع مشابه
A stability-Indicating HPLC Method for Simultaneous Determination of Creatine Phosphate Sodium and its Related Substances in Pharmaceutical Formulation
The objective of the study was to develop a simple, specific and stability-indicating HPLC method for the simultaneous determination of creatine phosphate sodium (CPS) and its related substances in pharmaceutical formulation. Separation of creatine phosphate sodium from its major process impurities and degradation products was achieved on a Hypersil BDS C18 column (250 × 4.6 mm, 5 μm) with an a...
متن کاملA stability-Indicating HPLC Method for Simultaneous Determination of Creatine Phosphate Sodium and its Related Substances in Pharmaceutical Formulation
The objective of the study was to develop a simple, specific and stability-indicating HPLC method for the simultaneous determination of creatine phosphate sodium (CPS) and its related substances in pharmaceutical formulation. Separation of creatine phosphate sodium from its major process impurities and degradation products was achieved on a Hypersil BDS C18 column (250 × 4.6 mm, 5 μm) with an a...
متن کاملThe direct colorimetric estimation of reducing sugars and other reducing substances with tetrazolium salts.
Since Kuhn & Jerchel (1941) showed that tetrazolium salts were reduced by bacteria, yeasts and germinating seeds, 2:3:5-triphenyltetrazolium chloride or bromide has come into prominence as a viability testing reagent (Cottrell, 1947) and for vital staining (Mattson, Jensen & Dutcher, 1947). Its specific advantages for this purpose and for the purposes which follow lie in its Eo value of -008 V....
متن کاملEvaluation of cotton fibers stickiness by colorimetric method
Stickiness is the physical process of contaminated lint adhering to equipment. The degree of stickiness depends on chemical identity, quantity, and distribution of the sugars. The sugar in cotton fibers may be insect or plant derived. There are several methods for measuring cotton fibers stickiness. In this work, a colorimetric method was used for the measurements. In the method, honeydew was c...
متن کاملChitosan Capped Silver Nanoparticles as Colorimetric Sensor for the Determination of Iron(III)
A selective, simple and low-cost method for the colorimetric determination of Fe3+ ions based on chitosan capped silver nanoparticles (Chit-AgNPs) was presented. Chitosan is a cationic polyelectrolyte and possesses amino and hydroxy groups which make it widely used as a capping agent for Ag NPs. The synthesized chitosan capped silver nanoparticles with excellent colloidal stability were charact...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2002